Correlation between antimicrobial resistance and virulence genes in Klebsiella pneumoniae isolates from Egypt

Objective: To genotypically assess the relationship between certain resistance and virulence determinants.Method: The cross-sectional study was conducted at Kafrelsheikh University, Egypt, from March 2019 to May 2021, comprised pathological samples, like blood, sputum, urine, vaginal swabs wound swabs, that had been taken patients who never received treatment. sample were collected Mansoura University hospitals, Klebsiella pneumoniae isolates obtained. Resistance determinants tested phenotypically. Uniplex polymerase chain reaction used evaluate presence of several resistanceaccompanied genes in isolates. Disc diffusion method isolates’ susceptibility accordance with Clinical Laboratory Standards Institute criteria for identifying diverseresistance patterns.Results: There 23 16 patients. Of isolates, 22(95.65%) showed drug resistance; 19(82.6%) multidrug resistance, 3(13.04%) extensive resistance. no case pan CTX-M-15, NDM, CTX-M-1, VIM-1 qnr B detected 14(60.86%), 13(56.5%), 6(26.08%), 6(26.08%) respectively. Moreover, exhibited extended-spectrum beta-lactamase producers, 12(52.17%) such contained both CTX-M-1 CTX-M-15 genes, 6 33.3% CTXM- 15 fox genes. Type 3 fimbriae adhesin mrkD mucoviscosity regulatory gene uge found testedisolates. However, iron uptake system kfu 8(34.78%) increased serum survival protein is accompanied magA A direct correlation among 5 8 antimicrobial genes.Conclusion: a existence factors antimicrobials.Keywords: Lactamase, pneumoniae, Anti-bacterial, Virulence, Bacterial, Genes.